Enhanced expression of an exocrine pancreatic protein in Alzheimer's disease and the developing human brain.
作者： S M de la Monte , M Ozturk , J R Wands
DOI: 10.1172/JCI114762
关键词:
摘要: Pancreatic thread protein (PTP) is a major exocrine secretory that in vitro forms filamentous bundles reminiscent of the paired helical filaments Alzheimer's disease (AD). We previously described increased PIP immunoreactivity AD brains and now report high levels developing human brain. Using full-length cloned bovine PTP cDNA synthetic oligonucleotides corresponding to cDNA, which identical islet cell regeneration factor, we analyzed expression ofPTP pancreas A 0.9-kb as well several minor transcripts were identified pancreas. In brain, same size detected by Northern analysis, primer extension assay, or polymerase chain reaction amplification cDNAs generated reverse transcriptase assay. There significantly higher mRNA with compared aged controls, amounts 1.2-, 0.6-, 0.4-kb analysis. situ hybridization localized pyramidal neurons cerebral cortex, population contains neurofibrillary tangles ofimmunoreactive PIP. These findings suggest thatAD associated enhanced PTP-related intraneuronal accumulation PTIP-like proteins. (J. Clin. Invest. 1990. 86:1004-1013.) Key words: pancreatic protein* * Down's syndrome central nervous system
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