Sensitive RP-HPLC Enantioseparation of (RS)-Ketamine via Chiral Derivatization Based on (S)-Levofloxacin

摘要: Enantioseparation of (RS)-ketamine has been achieved in the form its diastereomeric hydrazones. A new chiral reagent was synthesized from enantiomerically pure (S)-levofloxacin by converting carboxyl group into a hydrazide derivative: provided reaction site for ketonic present (RS)-ketamine. Because structural feature formation hydrazones successful without protection amino group. The were separated on reversed-phase C18 column with mobile phase consisting MeCN and 0.1% TFA under gradient elution 35 to 65% MeCN. limit detection found be 3.2 3.4 nmol first second eluting hydrazones, respectively. separation mechanism order proposed supported developing geometry optimized ‘lowest energy’ structures two using DFT-based Gaussian software.