Glioma big potassium channel expression in human cancers and possible T cell epitopes for their immunotherapy.

摘要: Big potassium (BK) ion channels have several spliced variants. One variant initially described within human glioma cells is the BK (gBK) channel. This isoform consists of 34 aa inserted into intracellular region basic PCR primers specific for this confirmed that cell lines and freshly resected surgical tissues from glioblastoma multiforme patients strongly expressed gBK mRNA. Normal brain tissue very weakly transcript. An Ab displayed protein in membrane, mitochondria, Golgi, endoplasmic reticulum. Within region, two putative epitopes (gBK1 gBK2) are predicted to bind HLA-A*0201 molecule. HLA-A*0201–restricted CTLs were generated vitro using peptide-pulsed dendritic cells. Both gBK1 gBK2 peptide-specific killed HLA-A2+/gBK+ gliomas, but they failed kill non-HLA-A2–expressing gBK+ target cytolytic assays. T2 loaded with exogenous peptides, not influenza M1 control peptide, only by their respective CTLs. The gBK-specific also a variety other HLA-A*0201+ cancer possess gBK, as well HLA-A2+ HEK transfected gene. Of clinical relevance, we found T derived sensitized peptide could expressing gBK. study shows peptides cancer-associated maybe useful targets cell-mediated immunotherapy.