Simultaneous Site-Specific Dual Protein Labeling Using Protein Prenyltransferases.
作者： Yi Zhang , Melanie J. Blanden , Ch. Sudheer , Soumyashree A. Gangopadhyay , Mohammad Rashidian
DOI: 10.1021/ACS.BIOCONJCHEM.5B00553
关键词:
摘要: Site-specific protein labeling is an important technique in chemistry and used for diverse applications ranging from creating conjugates to immobilization. Enzymatic reactions, including prenylation, have been widely exploited as methods accomplish site-specific labeling. prenylation catalyzed by prenyltransferases, farnesyltransferase (PFTase) geranylgeranyltransferase type I (GGTase-I), both of which recognize C-terminal CaaX motifs with different specificities transfer prenyl groups isoprenoid diphosphates their respective target proteins. A number analogues containing bioorthogonal functional label proteins interest via PFTase-catalyzed reaction. In this study, we sought expand the scope prenyltransferase-mediated exploring utility rat GGTase-I (rGGTase-I). First, specificity rGGTase-I was evaluated screening eight differen...
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