Chapter 17 Transmembrane Signaling in Dictyostelium

摘要: Publisher Summary Dictyostelium provides a biochemically and genetically accessible system for studies of transmembrane signaling. Current techniques to control cellular sensitivity monitor chemotaxis, cyclic adenosine monophosphate (cAMP) signaling, adenylate cyclase activation are outlined. Receptor-binding assays reviewed, protocol receptor purification is offered. The brought together in flow sheet characterization signaling mutants. Transmembrane mutants can be simply isolated by screening morphological aberrations during development. differentiated phenotype reversible so that, once isolated, the grown propagated as wild type. A cell surface cAMP identified purified, specific antiserum raised. Evidence suggests that this may regulate cyclase, gene expression. An extensive, cAMP-mediated phosphorylation cause adaptation physiological responses. recent demonstration vitro guanylnucleotide regulation mechanisms, similar those found vertebrates, operate primitive eukaryotic organism.