Distribution and axonal projections of neurons coexpressing thyrotropin-releasing hormone and urocortin 3 in the rat brain.

摘要: Thyrotropin-releasing hormone (TRH) decreases food intake when administered intracerebroventricularly or into the ventromedial hypothalamus. However, it is unknown which population of TRH neurons exerts this anorexigenic function. In rostral perifornical area, pattern TRH-expressing reminiscent distribution expressing urocortin3 (Ucn3) that also inhibits feeding injected hypothalamic nucleus (VMN). Since colocalization and Ucn3 may help to identify feeding-related neurons, putative coexpression two peptides was examined using fluorescent in situ hybridization combined with immunofluorescence. Almost all (95.5 +/- 0.2%) Ucn3-immunoreactive area expressed pro-TRH mRNA, while 50.2 1.6% were double-labeled bed stria terminalis (BNST). Only a few Ucn3/pro-TRH found outside these areas. The axons containing both by dual Ucn3/TRH fibers heavily innervated VMN. addition, high densities observed lateral septal nucleus, posterior division BNST, medial amygdaloid amygdalohippocampal ventral hippocampus, forebrain areas associated psychological stress anxiety. We conclude are coexpressed discrete, continuous making neurochemical marker define distinct subset neurons. their suggests coordinate behavioral responses stressful stimuli.