Shikimic acid inhibits LPS-induced cellular pro-inflammatory cytokines and attenuates mechanical hyperalgesia in mice.

摘要: Abstract Background and aims Shikimic acid (SA) is present in a wide variety of plants microorganisms used traditional folk medicine also an essential starting material for the synthesis antiviral drug Oseltamivir (Tamiflu®). Some pharmacological actions observed SA-enriched products include antioxidant anti-inflammatory activities. Here, we investigated antinociceptive isolated SA. Methods RAW 264.7 macrophage cells were treated with bacterial LPS (1 μg/mL) effect SA on modulation cell viability, nitric oxide (NO) production, TNF-α, IL-1β content MAPK (ERK1/2 p38) activation was evaluated. Besides, anti-hyperalgesic vivo model mechanical hyperalgesia induced by carrageenan (CG), dopamine (DA), TNF-α prostaglandin (PGE 2 ) assessed. Results In cells, suppressed LPS-induced decrease viability nitrite accumulation to control values inhibited up-regulation (65%) (39%). These effects may be mediated at least part inhibition ERK 1/2 (22%) p38 (17%) phosphorylation. mice, 50, 100, 200 mg/kg decreased formalin-induced nociceptive behavior (around 50%) inflammatory nociception PGE (50 75% each). Moreover, (100 200 mg/kg) significantly attenuated CG DA (25 40% Conclusions results indicate that presents potential development drugs treat pro-inflammatory painful conditions.