Tumor suppressor miR‐139‐5p targets Tspan3 and regulates the progression of acute myeloid leukemia through the PI3K/Akt pathway

摘要: Dysregulation of microRNAs is closely implicated in the initiation and progression human cancers including acute myeloid leukemia (AML). Though miR-139-5p was reported to be a potent tumor suppressor adult AML, its underlying molecular mechanism AML remains further defined. Herein, quantitative real-time polymerase chain reaction (qRT-PCR) Western blot analysis were conducted determine expressions tetraspanin3 (Tspan3) patients cells. Luciferase reporter assay, qRT-PCR, carried out detect interaction between Tspan3. Cell proliferation, cell cycle distribution, invasion, migration evaluated by counting kit-8, flow cytometry, transwell assays, respectively. phosphorylated-protein kinase B (Akt) Akt levels. We found that significant reduction expression prominent increase Tspan3 observed confirmed as direct target negatively modulated miR-139-5p. Rescue experiments showed overexpression constrained invasion capabilities, induced arrest at S phase cells, which partially reversed overexpression. In addition, we suppressed phosphoinositide 3-kinase (PI3K)/Akt pathway cells targeting conclusion, our study concluded leukemogenesis through inactivation PI3K/Akt pathway, providing better understanding progression.