Lipid metabolism of phenol-tolerant Rhodococcus opacus strains for lignin bioconversion.

摘要: Lignin is a recalcitrant aromatic polymer that potential feedstock for renewable fuel and chemical production. Rhodococcus opacus PD630 promising strain the biological upgrading of lignin due to its ability tolerate utilize lignin-derived compounds. To enhance tolerance, we recently applied adaptive evolution using phenol as sole carbon source characterized phenol-adapted R. (evol40) wild-type (WT) by whole genome RNA sequencing. While this effort increased our understanding tolerance mechanisms were not completely elucidated. We hypothesize composition lipids plays an important role in tolerance. test hypothesis, high-resolution mass spectrometry analysis lipid samples obtained from WT evol40 strains grown 1 g/L glucose (glucose), 0.75 g/L (low phenol), or 1.5 g/L (high phenol, only) source. This identified > 100 species mycolic acids, phosphatidylethanolamines (PEs), phosphatidylinositols (PIs), triacylglycerols. In both strains, acids had fewer double bond numbers conditions than condition, significantly shorter acid chain lengths conditions. These results indicate adaptation affected membrane composition. addition, percentage unsaturated phospholipids decreased compared condition. Moreover, PI content low condition further high relative work represents first comprehensive lipidomic study on Our suggest alteration phospholipid may be strategy