Immunohistochemical and in situ localization of Crimean-Congo hemorrhagic fever (CCHF) virus in human tissues and implications for CCHF pathogenesis.

摘要: Background Crimean-Congo hemorrhagic fever (CCHF) is a potentially fatal disease that occurs in parts of Africa, Asia, and eastern Europe, caused by recently emerged bunyavirus. Rapid laboratory diagnosis CCHF infection essential currently performed virus isolation serology. Histopathologic studies have been limited to small number cases, little known about the cellular tropism pathogenesis this disease. Design We conducted retrospective case analysis 12 patients with infection, confirmed isolation, who were evaluated at Special Pathogens Unit, National Institute for Virology, South Africa. The clinicopathologic features diagnostic role as compared serology, immunohistochemistry, situ hybridization evaluated. Additionally, distribution human tissues was examined. Results clinical histopathologic resemble those other viral fevers. Of isolation-confirmed 5 positive 10 hybridization. Immunohistochemistry analyses showed mononuclear phagocytes, endothelial cells, hepatocytes are main targets infection. Association parenchymal necrosis liver suggests cell damage may be mediated direct cytopathic effect. Conclusions CCHF, suspected history features, can supported histopathologically. However, since pathologic fevers, an unequivocal made only tests. utility immunohistochemistry sensitive rapid modality established high degree concordance isolation. Infection play critical CCHF.