Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection

摘要: Objective. Gelatin-thrombin matrix (GTM) tissue sealant use was previously identified as an independent predictor of pelvic infection following hysterectomies. We aim to elucidate contributing factors by assessing influence GTM on bacterial colony formation and characterizing bacteria present at the vaginal cuff. Methods. Escherichia coli incubated in phosphate-buffered saline (PBS) washings with without assess formation. Pelvic cuff were collected from hysterectomies occurring June through October 2015. In vitro techniques, 16S rRNA gene qPCR, amplicon sequencing performed characterize Results. Mean PBS greater for E. presence (1.48 × 10(7) CFU/mL) versus (9.95 10(5) CFU/mL) 20-hour incubation (p = 0.001). Out 61 samples, 3 culture positive (≥5000 CFU/mL) Enterococcus faecalis. Conclusion. experiments support a facilitating role PBS. However, given negative results surgical site adequate disinfection, promoting posthysterectomy infections may be limited. Analysis revealed faecalis, but inconclusive. Further studies are recommended.