Effects of recycled manure solids bedding on the spread of gastrointestinal parasites in the environment of dairies and milk.

摘要: The primary aim of this work was to isolate common bovine digestive tract parasites in recycled manure bedding (RMS), as well determine the ability current RMS preparation procedures eliminate these pathogens. Other objectives were assess whether any aforementioned could be retrieved bulk milk from dairies using and study prevalence differed among cows housed on versus straw bedding. For study, 27 farms 61 control recruited. Samples pre-pit tank recovered straw-bedding RMS-based farms. In addition, samples solid fraction after liquid extraction, before use, currently use herds. Parasites first detected by double centrifugation zinc sulfate flotation enhance isolation gastrointestinal protozoa, modified Wisconsin sugar for appraisal nematodes. Cryptosporidium confirmed nested PCR amplification sequencing a portion gene encoding small subunit rRNA. Results revealed high spp. (C. parvum, C. andersoni, meleagridis, identified PCR) Eimeria (mainly E. bovis zuernii) both types farms, with larger proportion RMS-bedded testing positive compared straw-bedded Both oocysts found at every step transformation, showing that strategies do not guarantee destruction protozoan parasites. potential zoonotic risk professionals close contact livestock, present 32 out 24 No sample derived milk, neither microscopy analysis nor molecular methods.