Imazalil exposure induces gut microbiota dysbiosis and hepatic metabolism disorder in zebrafish

摘要: The fungicide imazalil (IMZ) is used extensively to preserve freshness, prevent decay and control fungal infections in fruits, vegetables or other plants. Recently, some studies have reported that the real aquatic systems reached very high levels. Here, male adult zebrafish were exposed 100 1000μg/L IMZ for 1, 7, 21days, gut microbiota hepatic metabolism evaluated. Exposure a concentration of 21days decreased mucin secretion gut. Sequencing V3-V4 region bacterial 16S rRNA gene revealed significant increase diversity zebrafish. At phylum level, composition Proteobacteria Bacteroidetes was decreased, while those Fusobacteria Firmicutes increased after exposure 21days. genus 29 species microorganisms significantly changed exposure. Based on GC/MS metabolomics analysis, 101 metabolites observably altered IMZ-treatment group. These mainly associated with pathway glycolysis, amino acid metabolism, lipid metabolism. In addition, transcription genes related glycolysis including Aco, Cpt1, Acc1, Srebp1a Fas, liver when 7 results indicated could cause dysbiosis metabolic disorders