The Brucella suis virB operon is induced intracellularly in macrophages

摘要: A type IV secretion system similar to the VirB of phytopathogen Agrobacterium tumefaciens is essential for intracellular survival and multiplication mammalian pathogen Brucella. Reverse transcriptase-PCR showed that 12 genes encoding Brucella suis form an operon. Semiquantitative measurements virB mRNA levels by slot blotting transcription operon, but not flanking genes, regulated environmental factors in vitro. Flow cytometry used measure green fluorescent protein expression from promoter confirmed data blots. Fluorescence-activated cell sorter analysis fluorescence microscopy induced macrophages within 3 h after infection. Induction only occurred once bacteria were inside cells, phagosome acidification was shown be major signal inducing expression. Because Brucella, we suggest it triggers unknown effector molecules. These molecules play a role remodeling create unique compartment which replicates.