Suggestive evidence that the highly metastatic variant ESb of the T-cell lymphoma Eb is derived from spontaneous fusion with a host macrophage

摘要: Two lines of evidence are reported which suggest that the highly metastatic variant ESb T-cell lymphoma Eb is derived from spontaneous fusion with a host macrophage. Firstly, cells shown to express macrophage differentiation antigen Mac-1 was not found on or any other tumor tested except line Pu5. Secondly, progression low high capacity could be reproduced in vitro following hybridization thioguanine-resistant (EbTGR) syngeneic bone-marrow-derived macrophages. HAT medium-selected hybrid (Eb-F1 and Eb-F2) established. They were cell surface markers both parental lines: T lymphoid antigens T-lymphoma (Mac-1, class II MHC antigens) normal partner. The identified hybrids subclones thereof by means monoclonal antibodies 3 different detection assays: cytofluorography, complement-dependent cytotoxicity immunoprecipitation followed gel electrophoresis. Animals inoculated s.c. EbTGR developed local tumors but metastases survived for more than 40 days. In contrast, animals similarly Eb-F1 Eb-F2 quickly visceral organs died as early 10-14 days inoculation. many all respects, vitro-derived T-lymphoma-macrophage resembled vivo-derived ESb. These findings, together presence cells, (1) may cell, most likely (2) somatic an important mechanism genetic rearrangements leading variants. new under well-defined conditions, their subclones, become very useful tools studying contribution specific traits membrane-related structures various steps process.