Characterization of Stages in Development of Obesity-Diabetes Syndrome in Sand Rat (Psammomys obesus)

摘要: Sand rats ( Psammomys obesus ) maintained on a diet providing free choice between laboratory chow and salt bush Atriplex halimus were classified into four groups differing in extent of the diabetic syndrome: A, normoglycemic-normoinsulinemic; B, normoglycemichyperinsulinemic; C, hyperglycemic-hyperinsulinemic; or D, hyperglycemic with reduced insulin levels. The metabolic pattern these was characterized by measuring 1) uptake fatty acid-labeled, very-lowdensity lipoprotein-borne triglycerides (VLDL-TG) [ 3 H]%-2-deoxyglucose (2-DOG) muscle adipose tissues; 2) incorporation 14 C]alanine glycogen vivo; 3) gluconeogenesis from lactate, pyruvate, alanine hepatocytes; 4) effect synthesis glucose; 5) oxidation albumin-bound [1- C]palmitate C]glucose strips soleus muscle; 6) activities tissue lipoprotein lipase; 7) rate-limiting enzymes glycolysis, gluconeogenesis, acid liver. In group VLDL-TG activity lipase higher lower than albino rats. liver, phosphoenolpyruvate carboxykinase, as well lipid NAOP-malate dehydrogenase,were rats, whereas pyruvate kinase lower. similar A. Uptake 2-DOG liver carboxykinase C further increased. progressive decrease found, reduced. increased, reached maximum D. These results suggest that hyperinsulinemic stage (group B), glucose is reduced, but suppresses stimulates hepatic TG. Hyperglycemia manifests itself when resistance increased reduction peripheral uptake. characteristics can be regarded model for development type II diabetes humans evoked nutritional affluence.