Fibroblast Growth Factor 10-Fibroblast Growth Factor Receptor 2b Mediated Signaling Is Not Required for Adult Glandular Stomach Homeostasis
作者： Allison L. Speer , Denise Al Alam , Frederic G. Sala , Henri R. Ford , Saverio Bellusci
DOI: 10.1371/JOURNAL.PONE.0049127
关键词:
摘要: The signaling pathways that are essential for gastric organogenesis have been studied in some detail; however, those regulate the maintenance of epithelium during adult homeostasis remain unclear. In this study, we investigated role Fibroblast growth factor 10 (FGF10) and its main receptor, receptor 2b (FGFR2b), glandular stomach homeostasis. We first showed mouse expressed Fgf10, receptors, Fgfr1b Fgfr2b, most other FGFR2b ligands (Fgf1, Fgf7, Fgf22) except Fgf3 Fgf20. Fgf10 expression was mesenchymal whereas FGFR1 FGFR2 were mostly epithelial. Studying double transgenic mice allow inducible overexpression mice, normal increased epithelial proliferation, drove mucous neck cell differentiation, reduced parietal chief differentiation. Although a similar phenotype can be associated with development metaplasia, found short duration does not cause metaplasia. Finally, investigating soluble form FGF10's which acts as dominant negative, no significant changes proliferation or differentiation mutants. Our work provides evidence, time, FGF10-FGFR2b pathway is required
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