Interference with PSMB4 Expression Exerts an Anti-Tumor Effect by Decreasing the Invasion and Proliferation of Human Glioblastoma Cells.

摘要: Background/Aims: Glioblastoma (GBM) is a malignant brain tumor with poor prognosis. Proteasome subunit beta type-4 (PSMB4) an essential that contributes to the assembly of 20S proteasome complex. However, role PSMB4 in glioblastomas remains be clarified. The aim this study was investigate GBM progression. Methods: We first analyzed protein and mRNA expression 80 clinical specimens 77 datasets from National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database. Next, we inhibited by siRNA cellular animal models explore PSMB4’s underlying mechanisms. cell survival after siPSMB4 transfection assayed MTT assay. Annexin V propidium iodide staining used monitor apoptosis flow cytometric analysis. Moreover, migration invasion were evaluated wound healing Transwell assays. migration-related invasion-related proteins inhibition detected Western blotting. In addition, orthotropic xenograft mouse model assay effect knockdown vivo study. Results: Basis on results bioinformatics study, glioma patients higher had shorter time than those lower expression. tissues showed elevated compared normal tissues. decreased proliferation, abilities human cells. Downregulated resulted cycle arrest vitro . model, tumors progression reduced when down-regulated. enhanced anti-tumor temozolomide (TMZ) growth. absence phosphorylated focal adhesion kinase matrix metallopeptidase 9 Conclusion: combination TMZ may exert decreasing proliferation as well promoting glioblastoma This research improve therapeutic efficacy treatment.