A comparison of redox polymer and enzyme co-immobilization on carbon electrodes to provide membrane-less glucose/O2 enzymatic fuel cells with improved power output and stability

摘要: Abstract Glassy carbon and graphite electrodes modified with films of enzyme osmium redox polymer, cross linked poly (ethylene glycol) diglycidyl ether, were used for elaboration a glucose/O2 enzymatic fuel cell. The polymers [Os(4,4′-dimethoxy-2,2′-bipyridine)2(polyvinylimidazole)10Cl]+ [Os(4,4′-dichloro-2,2′-bipyridine)2(polyvinylimidazole)10Cl]+ selected to facilitate transfer electrons from the glucose oxidase (GOx) active site T1 Cu multicopper oxygenases Trametes hirsuta laccase (ThLacc) Myrothecium verrucaria bilirubin (MvBOD). Maximum power density at pH 5.5 3.5 μW cm−2 cell voltage 0.35 V was obtained an assembled membrane-less based on ThLacc glassy as cathode, in presence 0.1 M glucose, 37 °C, lower observed 7.4 4.5. Replacement cathode that MvBOD produced 10 μW cm−2 0.25 V under pseudo-physiological conditions. base electrode material resulted increased polymer loading, leading increase output 43 μW cm−2 similar Improved stabilization biofilms achieved through covalent anchoring electrodes, derivatized via electrochemical reduction diazonium cation generated situ p-phenylenediamine. Enzymatic cells using this approach retained 70% 24 h, whereas prepared without chemical only 10% over same interval.