Spectrofluorometric Analysis of Autofluorescing Components of Crude Serum from a Rat Liver Model of Ischemia and Reperfusion.
作者： Anna C. Croce , Andrea Ferrigno , Clarissa Berardo , Giovanni Bottiroli , Mariapia Vairetti
DOI: 10.3390/MOLECULES25061327
关键词:
摘要: Autofluorescence (AF) of crude serum was investigated with reference to the potential its intrinsic AF biomarkers for noninvasive diagnosis liver injury. Spectral parameters pure compounds representing retinol (vitamin A) and fluorescing free fatty acids were characterized by spectrofluorometry, assess spectral subsequent analysis serum, collected from rats undergoing ischemia/reperfusion (I/R). Differences in profiles detected between control I/R due increase components samples. No significant changes occurred levels, consistently literature reporting that constant levels are commonly observed blood, except malnutrition or chronic severe disease. Conversely, acids, particular arachidonic linoleic acid their derivatives, act as modulating agents inflammation, both a protective damaging response stress stimuli. The biometabolic pathophysiological meaning possibility direct detection spectrofluorometry open up interesting perspectives development analysis, direct, cost effective, supportive tool injury related systemic metabolic alterations, applications experimental biomedicine foreseen translation clinics.
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