Proteomic analysis of the phytopathogenic soilborne fungus Verticillium dahliae reveals differential protein expression in isolates that differ in aggressiveness.

摘要: Verticillium dahliae is a soilborne fungus that causes vascular wilt disease of plants and losses in broad range economically important crops worldwide. In this study, we compared the proteomes highly (Vd1396-9) weakly (Vs06-14) aggressive isolates V. to identify protein factors may contribute pathogenicity. Twenty-five spots were consistently observed as differential proteome profiles two isolates. The sequences identified by LC-ESI-MS/MS MASCOT database searches. Some shared homology with fungal proteins have roles stress response, colonization, melanin biosynthesis, microsclerotia formation, antibiotic resistance, penetration. These are functions for infection host survival pathogen soil. One found only isolate was isochorismatase hydrolase, potential plant-defense suppressor. This enzyme inhibit production salicylic acid, which plant defense response signaling. Other corresponding pathogenicity isolate. work indicates that, combination functional genomics, proteomics-based analyses can provide additional insights into pathogenesis management strategies disease.