Phenotypes and functional effects caused by various viral RNA silencing suppressors in transgenic Nicotiana benthamiana and N. tabacum.
作者： Shahid Aslam Siddiqui , Cecilia Sarmiento , Erkki Truve , Harry Lehto , Kirsi Lehto
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摘要: RNA silencing suppressor genes derived from six virus genera were transformed into Nicotiana benthamiana and N. tabacum plants. These suppressors P1 of Rice yellow mottle (RYMV), Cocksfoot virus, P19 Tomato bushy stunt P25 Potato X, HcPro Y (strain N), 2b Cucumber mosaic Kin), AC2 African cassava (ACMV). caused the most severe phenotypes in both spp. also produced effects but a much milder phenotype benthamiana, although affected leaf tissues two similar ways, causing hyperplasia hypoplasia, respectively. P1-RYMV high lethality plants only mild Phenotypic alterations by other transgenes minor species. Interestingly, had very different on crucifer-infecting Tobamovirus (crTMV) infections. enhanced spread brightness crTMV-green fluorescent protein (GFP) lesions, whereas not these lesions. promoted infection new foci within infiltrated leaf, suppressed crTMV-GFP
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sci-hub.se 参考文章(53)
David P Bartel, MicroRNAs: Genomics, Biogenesis, Mechanism, and Function Cell. ,vol. 116, pp. 281- 297 ,(2004) , 10.1016/S0092-8674(04)00045-5
Y. Kurihara, Y. Watanabe, Arabidopsis micro-RNA biogenesis through Dicer-like 1 protein functions Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 12753- 12758 ,(2004) , 10.1073/PNAS.0403115101
Mathew Lewsey, Fiona C. Robertson, Tomas Canto, Peter Palukaitis, John P. Carr, Selective targeting of miRNA-regulated plant development by a viral counter-silencing protein. Plant Journal. ,vol. 50, pp. 240- 252 ,(2007) , 10.1111/J.1365-313X.2007.03042.X
Wonkeun Park, Junjie Li, Rentao Song, Joachim Messing, Xuemei Chen, CARPEL FACTORY, a Dicer Homolog, and HEN1, a Novel Protein, Act in microRNA Metabolism in Arabidopsis thaliana Current Biology. ,vol. 12, pp. 1484- 1495 ,(2002) , 10.1016/S0960-9822(02)01017-5
S. A. Siddiqui, C. Sarmiento, S. Valkonen, E. Truve, K. Lehto, Suppression of Infectious TMV Genomes Expressed in Young Transgenic Tobacco Plants Molecular Plant-microbe Interactions. ,vol. 20, pp. 1489- 1494 ,(2007) , 10.1094/MPMI-20-12-1489
Matthew W. Rhoades, Brenda J. Reinhart, Lee P. Lim, Christopher B. Burge, Bonnie Bartel, David P. Bartel, Prediction of Plant MicroRNA Targets Cell. ,vol. 110, pp. 513- 520 ,(2002) , 10.1016/S0092-8674(02)00863-2
Zsuzsanna Mérai, Zoltán Kerényi, Attila Molnár, Endre Barta, Anna Válóczi, György Bisztray, Zoltán Havelda, József Burgyán, Dániel Silhavy, Aureusvirus P14 is an efficient RNA silencing suppressor that binds double-stranded RNAs without size specificity. Journal of Virology. ,vol. 79, pp. 7217- 7226 ,(2005) , 10.1128/JVI.79.11.7217-7226.2005
Masoud Shams-Bakhsh, Tomas Canto, Peter Palukaitis, Enhanced resistance and neutralization of defense responses by suppressors of RNA silencing Virus Research. ,vol. 130, pp. 103- 109 ,(2007) , 10.1016/J.VIRUSRES.2007.05.023
Qi Xie, Hui-Shan Guo, Systemic antiviral silencing in plants. Virus Research. ,vol. 118, pp. 1- 6 ,(2006) , 10.1016/J.VIRUSRES.2005.11.012
Cecilia Sarmiento, Eva Gomez, Merike Meier, Tony A. Kavanagh, Erkki Truve, Cocksfoot mottle virus P1 suppresses RNA silencing in Nicotiana benthamiana and Nicotiana tabacum Virus Research. ,vol. 123, pp. 95- 99 ,(2007) , 10.1016/J.VIRUSRES.2006.07.013