High-Throughput Cloning of Campylobacter jejuni ORFs by in Vivo Recombination in Escherichia coli

摘要: A rate-limiting and costly step in many proteomics analyses is the cloning of all ORFs for an organism into technique-specific vectors. Here, we describe generation a Campylobacter jejuni expression clone set using high-throughput approach based on recombination E. coli. The uses native coli functions requires no vitro enzymatic steps or special strains. Our results indicate that this efficient economical alternative cloning.