Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications
作者： Timothy E.L. Douglas , Agata Łapa , Sangram Keshari Samal , Heidi A. Declercq , David Schaubroeck
DOI: 10.1002/TERM.2273
关键词:
摘要: Mineralization of hydrogel biomaterials is considered desirable to improve their suitability as materials for bone regeneration. Calcium carbonate (CaCO3 ) has been successfully applied a regeneration material, but hydrogel-CaCO3 composites have received less attention. Magnesium (Mg) used component calcium phosphate stimulate bone-forming cell adhesion and proliferation in vivo, its effect carbonate-based remains uninvestigated. In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched magnesium generate composite Hydrogels loaded enzyme urease by incubation mineralization media containing urea different ratios ions. Increasing concentration decreased mineral crystallinity. At low concentrations calcite was formed, while at higher magnesian formed. Hydromagnesite (Mg5 (CO3 )4 (OH)2 .4H2 O) formed high absence calcium. The amount compressive strength increasing medium. calcium:magnesium elemental ratio than respective media. or promoted growth osteoblast-like cells. hydromagnesite displayed cytotoxicity. conclusion, enzymatic GG form reinforced growth, enrichment had no definitive positive effect. Copyright © 2017 John Wiley & Sons, Ltd.
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