A new and simple technique of total hepatic ischemia in the mouse.

摘要: Background. A model of total hepatic ischemia is currently not available in mice. Models described rats using portosystemic shunts to achieve have been notoriously difficult. In mice, the problem compounded further when this type technique because small size animal. new combining partial hepatectomy with clamping remnant liver. Methods. (30%) performed resection caudate, right lateral, and quadrate lobes, papillary process. Vascular microclamps are placed across pedicles median left lateral lobe at level hilum ischemia. Spontaneous portocaval through caudate branches collateral vessels prevent mesenteric congestion. Animals were studied for survival. Results. The procedure consistently took less than 30 min (25±2 min), no bleeding resected tissue was observed. Evidence spontaneous demonstrated by use an intraportal dye. All animals survived 60 ischemia, whereas all died after 90 Conclusion. This a technically simple rapid perform. current environment multiple knockout mice bioreagents that available, essential.