Calcium imaging of single stereocilia in hair cells: Localization of transduction channels at both ends of tip links
作者： Winfried Denk , Jeffrey R. Holt , Gordon M.G. Shepherd , David P. Corey
DOI: 10.1016/0896-6273(95)90010-1
关键词:
摘要: Abstract Mechanically gated "transduction" channels in inner ear hair cells are thought to be connected tip links stretched between adjacent stereocilia. To locate active channels, calcium-green fluorescence single stereocilia was measured with two-photon laser scanning microscopy. Bundle deflection increased many but not all stereocilia; the increase blocked by depolarization. The number of responding proportional transduction current, consistent Ca 2+ influx through channels. Fluorescence rose first tips and then bases, agreement channel localization at tips. Some shortest a bundle showed increase, as did some tallest, indicating that can either or both ends links.
core.ac.uk
elsevier.com
sci-hub.se 参考文章(44)
Winfried Denk, David W. Piston, Watt W. Webb, Two-photon molecular excitation in a laser scanning microscopy Springer US. pp. 445- 458 ,(1990) , 10.1007/978-1-4757-5348-6_28
David K. Lynch, Theory and practice of scanning optical microscopy Journal of The Optical Society of America A-optics Image Science and Vision. ,vol. 4, pp. 551- 560 ,(1984) , 10.1364/JOSAA.4.000558
AJ Hudspeth, Extracellular current flow and the site of transduction by vertebrate hair cells The Journal of Neuroscience. ,vol. 2, pp. 1- 10 ,(1982) , 10.1523/JNEUROSCI.02-01-00001.1982
H Ohmori, Mechanical stimulation and Fura-2 fluorescence in the hair bundle of dissociated hair cells of the chick. The Journal of Physiology. ,vol. 399, pp. 115- 137 ,(1988) , 10.1113/JPHYSIOL.1988.SP017071
PA Fuchs, BW Murrow, Cholinergic inhibition of short (outer) hair cells of the chick's cochlea The Journal of Neuroscience. ,vol. 12, pp. 800- 809 ,(1992) , 10.1523/JNEUROSCI.12-03-00800.1992
DP Corey, AJ Hudspeth, Kinetics of the receptor current in bullfrog saccular hair cells The Journal of Neuroscience. ,vol. 3, pp. 962- 976 ,(1983) , 10.1523/JNEUROSCI.03-05-00962.1983
GM Shepherd, DP Corey, The extent of adaptation in bullfrog saccular hair cells The Journal of Neuroscience. ,vol. 14, pp. 6217- 6229 ,(1994) , 10.1523/JNEUROSCI.14-10-06217.1994
J. Howard, A.J. Hudspeth, Compliance of the hair bundle associated with gating of mechanoelectrical transduction channels in the Bullfrog's saccular hair cell Neuron. ,vol. 1, pp. 189- 199 ,(1988) , 10.1016/0896-6273(88)90139-0
Rafael Yuste, Winfried Denk, Dendritic spines as basic functional units of neuronal integration. Nature. ,vol. 375, pp. 682- 684 ,(1995) , 10.1038/375682A0
Peter G. Gillespie, Mark C. Wagner, A.J. Hudspeth, Identification of a 120 kd hair-bundle myosin located near stereociliary tips Neuron. ,vol. 11, pp. 581- 594 ,(1993) , 10.1016/0896-6273(93)90071-X