20-Hydroxyecdysone is required for, and negatively regulates, transcription of Drosophila pupal cuticle protein genes.
作者： R.T. Apple , James W. Fristrom
DOI: 10.1016/0012-1606(91)90257-4
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摘要: Abstract Transcripts of ecdysone-dependent genes (EDGs) accumulate in isolated imaginal discs within 8 hr after exposure to a pulse the steroid hormone 20-hydroxyecdysone (20-HE; 1 μg/ml for 6 hr) but not cultured continuous presence or absence hormone. Sequence analyses show that two EDGs are members gene families encoding insect cuticle proteins. We conclude third EDG encodes protein because conceptual glycine-rich contains sequence motifs similar those found egg shell proteins and vertebrate cytokeratins expression this is limited tissues deposit pupal cuticle. Nuclear run-on assays hormone-dependent each these due transcriptional regulation. Readdition actively synthesizing messages causes rapid repression transcription. Thus, 20-HE acts as both positive negative regulator Sequences promoter regions an ecdysone response element may play role
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