Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers

摘要: Eryngium alpinum L. is an endangered species found across the European Alps. In order to obtain base-line data for conservation of this species, we investigated levels genetic diversity within and among 14 populations from French We used amplified fragment length polymorphism (AFLP) technique with three primer pairs scored a total 62 unambiguous, polymorphic markers in 327 individuals. Because AFLP are dominant, within-population structure (e.g. FIS) could not be assessed. Analyses based either on assumption random-mating or complete selfing lead very similar results. Diversity were relatively high (mean Nei's expected heterozygosity = 0.198; mean Shannon index 0.283), positive correlation was detected between both measurements population size (Spearman rank correlation: P 0. 005 0.002, respectively). Moreover, FST values exact tests differentiation revealed pairwise 0.40), which appeared independent geographical distance (nonsignificant Mantel test). Founder events during postglacial colonizations and/or bottlenecks proposed explain but random differentiation. By contrast, pattern isolation by valleys. Predominant local gene flow pollen seed probably responsible pattern. Concerning management E. alpinum, leads us recommend maximum number populations. This study demonstrates that enable quick reliable assessment intraspecific variability genetics.