Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways
作者： Mohan Babu , J. Javier Díaz-Mejía , James Vlasblom , Alla Gagarinova , Sadhna Phanse
DOI: 10.1371/JOURNAL.PGEN.1002377
关键词:
摘要: As the interface between a microbe and its environment, bacterial cell envelope has broad biological clinical significance. While numerous biosynthesis genes pathways have been identified studied in isolation, how these intersect functionally to ensure integrity during adaptive responses environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens generate quantitative functional association maps encompassing virtually entire biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) prototrophic (minimal culture conditions. The differential patterns interactions detected among > 235,000 digenic mutant combinations tested reveal unexpected condition-specific crosstalk backup mechanisms that stress-resistant assembly maintenance. These networks also provide insights into global systems connectivity dynamic reorganization universal structure is broadly conserved eubacteria (including pathogens) an important target.
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