The conversion of L-phenylalanine into benzoic acid on the thylakoid membrane of higher plants.

摘要: The conversion of L-phenylalanine into benzoic acid and other aromatic carboxylic acids was investigated in Nasturtium officinale (watercress), Astilbe chinensis, Hydrangea macrophylla vivo vitro. Comparative feeding experiments with radioactively labelled cinnamic administered to intact leaf discs A. chinensis indicated a rapid formation from L-phenylalanine, whereas poor precursor. Using pulse-chase labelling technique followed by fractionation the tissue subcellular components, chloroplasts could be identified as predominant, if not exclusive, site chinensis. Experiments vitro thylalkoids N. officinale, H. macrophylla, demonstrate capacity thylakoid membranes catalyze degradation acid. results obtained upon stimultaneous incubation [4'-3H]L-phenylalanine [3-14C]cinnamic lead hypothesis that reaction proceeds via pool which is different soluble