Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 1–34) with Biotinylated Peptide as Tracer

摘要: Along with calcitonin and 1,25-dihydroxyvitamin D3 the polypeptide parathyroid hormone (parathyrin) (PTH) is responsible for maintaining calcium phosphorus homeostasis in vertebrates. Parathyrin a of 84 amino acids produced by glands [1]. A change 0.2 g/dl blood concentration alters secretory state parathyoid [2]. The cleavage intact PTH into amino-terminal, midregional, carboxy-terminal fragments takes place liver kidneys [3–5]. Besides only amino-terminal part shows bioactivity. All other are biologically inert [6]. PTH(1–34) interacts directly bone and—indirectly—with intestine. This interaction increases level human serum [1, 7, 8]. diseases primary hyperparathyroi-dism (pHPT), secondary HPT, hypoparathyroidism closely connected secretion [8]. Detection levels radioimmunological procedures can provide diagnosis these diseases. Several homologous radioimmunoassays (RIAs) have been developed detection different [9–13]. To study chronic to diagnose hyper-parathyroidism RIA useful [13]. Human parathyrin hPTH (53–84) has half-life 1–2h [14]. It possible estimate function transplanted kidney measuring midregion PTH.