Influence of culture time on the expression of drug-metabolizing enzymes in primary human hepatocytes and hepatoma cell line HepG2.

摘要: Primary cultures of human hepatocytes and hepatoma cell line HepG2 are frequently used to evaluate the hepatic disposition drugs other xenobiotics. To check variability expression drug-metabolizing enzymes in these vitro models, genes coding for several cytochrome P450 isoforms phase II was quantified during culture time by real-time RT-PCR. Gene determined daily primary maintained a sandwich over 1 week HepG2, first 10 passages. In characteristic trends were observed which could be abstracted into three major classes curves. Genes second class had an maximum around day 6 4 culture, respectively. The third two peaks: at 5 culture. Surprisingly, also showed significant changes For example, gene 1A1 varied 8-fold, that 2B6 30-fold, NADP-quinone reductase more than 200-fold within conclusion, neither nor display model constant enzymes. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:207–213, 2003; Published online InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10085