Huaier aqueous extract protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting NLRP3 inflammasome activation.

摘要: // Lijuan Wang 1, * , Zhongxia Yu 2, Chao Wei 3, Li Zhang 4 Hui Song 2 Bing Chen 1 Qifeng Yang Pathology Tissue Bank, Qilu Hospital, Shandong University, Jinan, 250012, China Department of Immunology and Key Laboratory Infection Immunity Province, University School Medicine, 3 Ophthalmology, Second Hospital 250033, Breast Surgery, These authors contributed equally to this work Correspondence to: Yang, email: qifengy_sdu@163.com Keywords: Huaier, colitis, NLRP3, inflammasome, IL-1β Received: September 30, 2016      Accepted: March 15, 2017      Published: 23, 2017 ABSTRACT The use Trametes robiniophila Murr. (Huaier) as a complementary therapy for cancer has recently become increasingly common in China. However, whether Huaier can regulate host immune responses, especially innate immunity, remains largely unknown. NLRP3 inflammasome is multimeric complex consisting ASC caspase-1. inflammasomes respond variety endogenous (damage-associated molecular patterns) exogenous (pathogen-associated stimuli, play crucial roles defense against pathogens multiple diseases such ulcerative colitis (UC). In study, we investigated the anti-inflammatory effect dextran sulfate sodium (DSS)-induced murine revealed underlying mechanisms by targeting inflammasomes. C57BL/6 mice, oral administration attenuated DSS-induced colon shortening colonic pathological damage. Furthermore, analyzed on activation macrophages. inhibited activation-induced secretion caspase-1 cleavage. Moreover, decreased protein expression via promoting degradation through autophagy lysosome pathway. Therefore, our findings demonstrate novel function regulation suggest potential role inflammasome-associated diseases.