Role of heparin‐binding EGF‐related peptides in proliferation and apoptosis of activated ras‐stimulated intestinal epithelial cells
作者： Shinichiro Zushi , Yasuhisa Shinomura , Tatsuya Kiyohara , Yoshiji Miyazaki , Shusaku Tsutsui
DOI: 10.1002/(SICI)1097-0215(19971210)73:6<917::AID-IJC26>3.0.CO;2-#
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摘要: The ras mutation is a common and critical step in carcinogenesis. Autocrine growth factors are also known to play an important role cancer cell transformation. However, the contribution of autocrine regulation proliferation apoptosis activated ras-stimulated intestinal epithelium not fully understood. Therefore, we constructed ras-transfected epithelial clones (IEC-ras) examine epidermal factor (EGF)-related peptides behavior IEC-ras. Overexpression EGF family (transforming alpha, heparin-binding EGF-like factor, amphiregulin betacellulin) stronger phosphorylation receptor was observed IEC-ras compared with control cells. proliferated more rapidly than cells, specific kinase inhibitor, AG 1478, abolished increased Heparitinase chlorate prevented Additionally, expressed bcl-2 resistant induction by UV radiation mitomycin C. 1478 suppressed expression inhibited resistance had effects similar those 1478. Our data indicate that both
sci-hub.se 参考文章(32)
B.L. Ziober, J.K. Willson, L.E. Hymphrey, K. Childress-Fields, M.G. Brattain, Autocrine transforming growth factor-alpha is associated with progression of transformed properties in human colon cancer cells. Journal of Biological Chemistry. ,vol. 268, pp. 691- 698 ,(1993) , 10.1016/S0021-9258(18)54207-1
Francis M. Giardiello, Barbara A. Zehnbauer, Pankaj J. Pasricha, Richard J. Jones, Atul Bedi, James P. Barber, Stanley R. Hamilton, Gauri C. Bedi, Adil J. Akhtar, Inhibition of apoptosis during development of colorectal cancer. Cancer Research. ,vol. 55, pp. 1811- 1816 ,(1995)
E Yang, SJ Korsmeyer, Molecular thanatopsis: a discourse on the BCL2 family and cell death Blood. ,vol. 88, pp. 386- 401 ,(1996) , 10.1182/BLOOD.V88.2.386.BLOODJOURNAL882386
G E Gallick, L C Yeoman, M Blick, R J Coffey, S Untawale, D M Wildrick, M A Zorbas, M Olive, C P Hodgson, S M North, Transforming growth factor-alpha production and autoinduction in a colorectal carcinoma cell line (DiFi) with an amplified epidermal growth factor receptor gene. Cancer Research. ,vol. 53, pp. 1630- 1636 ,(1993)
G D Plowman, J M Green, V L McDonald, M G Neubauer, C M Disteche, G J Todaro, M Shoyab, The amphiregulin gene encodes a novel epidermal growth factor-related protein with tumor-inhibitory activity. Molecular and Cellular Biology. ,vol. 10, pp. 1969- 1981 ,(1990) , 10.1128/MCB.10.5.1969
F. Ciardiello, N. Kim, T. Saeki, R. Dono, M. G. Persico, G. D. Plowman, J. Garrigues, S. Radke, G. J. Todaro, D. S. Salomon, Differential expression of epidermal growth factor-related proteins in human colorectal tumors. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 7792- 7796 ,(1991) , 10.1073/PNAS.88.17.7792
Kathleen Forrester, Concepcion Almoguera, Kyuhyung Han, William E. Grizzle, Manuel Perucho, Detection of high incidence of K- ras oncogenes during human colon tumorigenesis Nature. ,vol. 327, pp. 298- 303 ,(1987) , 10.1038/327298A0
H Marquardt, M. Hunkapiller, L. Hood, G. Todaro, Rat transforming growth factor type 1: structure and relation to epidermal growth factor Science. ,vol. 223, pp. 1079- 1082 ,(1984) , 10.1126/SCIENCE.6320373
Eric R. Fearon, Bert Vogelstein, A genetic model for colorectal tumorigenesis Cell. ,vol. 61, pp. 759- 767 ,(1990) , 10.1016/0092-8674(90)90186-I
W. E. Carson, S. Haldar, R. A. Baiocchi, C. M. Croce, M. A. Caligiuri, The c-kit ligand suppresses apoptosis of human natural killer cells through the upregulation of bcl-2 Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 7553- 7557 ,(1994) , 10.1073/PNAS.91.16.7553