Purification and characterization of a lectin from rice bran.

摘要: A rice bran lectin was purified to homogeneity by precipitation with ammonium sulfate and chromatography on ovomucoid-Sepharose CM-cellulose. The molecular weight of the dimer estimated be around 37,000 ultracentrifugation studies. sedimentation coefficient 3.8S. On Sepharose 6B gel filtration in presence 6 M guanidine-HCl, showed a 19,000. reduction carboxymethylation, further dissociated into two nonidentical subunits, weights about 11,000 8,000. These subunits did not show hemagglutinating activity. Equilibrium dialysis experiments using N-acetyl-[1-14C]glucosamine indicated that 1.8 mol sugar bound 19,000 g lectin. mitogenic against mouse splenic lymphocytes human peripheral lymphocytes. enhanced rate glucose oxidation inhibited epinephrine-stimulated lipolysis adipocytes. Some characteristics are compared those wheat germ agglutinin.