Disposable urea biosensor based on nanoporous ZnO film fabricated from omissible polymeric substrate.
作者： Reza Rahmanian , Sayed Ahmad Mozaffari , Mohammad Abedi
DOI: 10.1016/J.MSEC.2015.08.004
关键词:
摘要: In the present study, a facile and simple fabrication method of semiconductor based urea biosensor was reported via three steps: (i) producing ZnO-PVA composite film by means polymer assisted electrodeposition zinc oxide (ZnO) on F-doped SnO2 conducting glass (FTO) using water soluble polyvinyl alcohol (PVA), (ii) obtaining nanoporous ZnO PVA omission subsequent post-treatment annealing film, (iii) preparation FTO/ZnO/Urs exploiting as an efficient excellent platform area for electrostatic immobilization urease enzyme (Urs) which forced difference in their isoelectric point (IEP). The characterization techniques focused analysis surfaces before after annealing, had prominent effect porosity prepared film. surface nanostructured field emission-scanning electron microscopy (FE-SEM), exhibited effective bio-sensing matrix immobilization. structural monitoring performed UV-Vis, Fourier Transform Infrared (FT-IR), Raman Spectroscopy, Thermogravimetric Analysis (TGA), Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) techniques. impedimetric results showed high sensitivity detection within 8.0-110.0mg dL(-1) with limit 5.0mg dL(-1).
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