Epidemiological study of Norwalk virus infections in Japan and Southeast Asia by enzyme-linked immunosorbent assays with Norwalk virus capsid protein produced by the baculovirus expression system

摘要: In this study, we investigated Norwalk virus (NV) antigen and antibody to recombinant NV (rNV) in human populations Japan Southeast Asia by enzyme-linked immunosorbent assays (ELISAs). Baculovirus-expressed capsid protein was used for preparing antisera rNV or as an detecting rNV. The ELISAs were specific had sensitivities equivalent higher than those of the previously developed radioimmunoassays. 159 stool samples obtained from children, mainly younger 10 years old, with acute gastroenteritis due small round structured viruses Japan, only 1 positive antigen. pattern acquisition quite different antibodies group A rotavirus calicivirus Sapporo (HuCV-Sa) strain. prevalence remained at a low level throughout childhood then showed steep rise during school age early adulthood Japan. high observed collected healthy adults Asia. These results suggested that infection is common but may be rare infants HuCV-Sa strain negative ELISA, no serological relationship between found. strain, although both are classified family Caliciviridae.