IMPROVED FLUORESCENT ASSAY SENSITIVITY USING SILVER ISLAND FILMS: FLUORESCEIN ISOTHIOCYANATE-LABELED ALBUMIN AS AN EXAMPLE
作者： OS Kulakovich , ND Strekal’ , MV Artem’ev , AP Stupak , SA Maskevich
DOI: 10.1007/S10812-006-0172-3
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摘要: UDC 543.426:547.962.3 We have studied enhancement of the fluorescence fluorescein isothiocyanate (FITC), bound to albumin and near an annealed silver island film, as a function distance between protein molecules metal. As intermediate spacer layer substrate, we used multilayer films based on polyelectrolytes. The maximum nine- fold coefficient for FITC corresponds thickness ≈4 nm, or three layers polyelectrolyte. In this case, observe significant decrease in average photoluminescence decay time label film compared with dielectric medium.
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