Thiol-ene Clickable Poly(glycidol) Hydrogels for Biofabrication.
作者： Simone Stichler , Tomasz Jungst , Martha Schamel , Ilona Zilkowski , Matthias Kuhlmann
DOI: 10.1007/S10439-016-1633-3
关键词:
摘要: In this study we introduce linear poly(glycidol) (PG), a structural analog of poly(ethylene glycol) bearing side chains at each repeating unit, as polymer basis for bioink development. We prepare allyl- and thiol-functional PG that can rapidly be polymerized to three-dimensionally cross-linked hydrogel network via UV mediated thiol-ene click reaction. Influence concentration irradiation on mechanical properties swelling behavior was examined. Thiol-functional synthesized in two variations, one containing ester groups are susceptible hydrolytic cleavage, the other ester-free stable against hydrolysis. This allowed preparation degradable non-degradable hydrogels. Cytocompatibility demonstrated by encapsulation human bone marrow-derived mesenchymal stem cells (hBMSCs). Rheological hydrogels were adjusted dispense plotting addition high molecular weight hyaluronic acid. The optimized formulation enabled highly reproducible constructs composed 20 layers with an overall height 3.90 mm.
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