Genomic Analysis of Antimicrobial Resistance Genotype-to-Phenotype Agreement in Helicobacter pylori.

摘要: Antimicrobial resistance (AMR) in Helicobacter pylori is increasing and can result treatment failure inappropriate antibiotic usage. This study used whole genome sequencing (WGS) to comprehensively analyze the H. resistome phylogeny order characterize Israeli pylori. isolates (n = 48) underwent antimicrobial susceptibility testing (AST) against five antimicrobials WGS analysis. Literature review identified 111 mutations reported correlate with phenotypic these antimicrobials. Analysis was conducted via our in-house bioinformatics pipeline targeting point relevant genes (pbp1A, 23S rRNA, gyrA, rdxA, frxA, rpoB) assess genotype-to-phenotype correlation. Resistance rates of were as follows: clarithromycin 54%, metronidazole 31%, amoxicillin 10%, rifampicin 4%, levofloxacin 2%. Genotype-to-phenotype correlation inconsistent; for every analyzed gene at least one phenotypically susceptible isolate found have a mutation previously associated resistance. also observed regarding commonly commercial kits diagnose AMR cases. Furthermore, 11 novel resistant phenotype detected. unique set demonstrates that inferring phenotypes from remains challenging should be optimized further.