Micropropagation of chestnut rose (Rosa roxburghii Tratt) and assessment of genetic stability in in vitro plants using RAPD and AFLP markers
作者： X. P. Wen , X. X. Deng
DOI: 10.1080/14620316.2005.11511891
关键词:
摘要: SummaryChestnut rose (Rosa roxburghii Tratt) shows desirable prospects both in fruit exploitation and ornamental plant genetic improvement. A micropropagation system based on axillary bud proliferation was established optimized for mature trees. Nodal segments, 30–45 d-old, gave the best regeneration after being incubated half-strength MS medium supplemented with gibberellic acid (GA3; 2.9 µM), 6-benzyladenine (BA; 2.2 naphthalene acetic (NAA; 0.5 activated charcoal (AC; 300–400 mg l–1). The multiple-shoot response obtained ‘Guinong No. 5’ ‘white-flower’ chestnut a modified MS1 µM GA3, 2.2–4.4 BA, NAA. Shoots 4–5 cm height, cultured 20–25 d 1/2 1.6 NAA, 2.5–3.5 paclobutrazol, l–1 AC showed rooting. No polymorphic marker found among 669 RAPD markers generated 124 arbitrary primers, multiplications of 5’. To test further t...
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