miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation

摘要: Human pluripotent stem cells retain the extraordinary capacity to differentiate into pancreatic beta cells. For this particular lineage, more effort is still required stress importance of developing an efficient, reproducible, easy, and cost-effective differentiation protocol obtain mature, homogeneous, functional insulin-secreting In addition, microRNAs (miRNAs) have emerged as a class small non-coding RNAs that regulate many cellular processes, including differentiation. Some miRNAs are known be preferentially expressed in islets. Of note, miR-375 miR-7 two most abundant miRNAs, they necessary for proper islet development. Here we provide new insight specific involved We found differentially during human embryonic (hESCs) cell-like phenotype its modulation plays important role generating mature This strategy may exploited optimize potential in vitro hESCs insulin-producing beta-like use preclinical studies future clinical applications well prospective uses improve process.