Methods, Compositions, and Kits for Detecting Allelic Variants

摘要: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, invention provides quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions deletions, samples comprising abundant wild type) variants with high specificity selectivity. particular, a highly selective method mutation detection referred competitive allele-specific TaqMan PCR (“cast-PCR”).