Microbial oxidation of amines. Partial purification of a trimethylamine mono-oxygenase from Pseudomonas aminovorans and its role in growth on trimethylamine

摘要: 1. A mono-oxygenase, which oxidizes trimethylamine and other tertiary amines bearing methyl or ethyl groups, was partially purified sixfold from Pseudomonas aminovorans grown on as sole carbon source. 2. The preferred electron donor NADPH. enzyme had a pH optimum of 8.0–9.4 for oxidation, 8.8–9.2 dimethylamine oxidation. 3. oxidation product shown to be N-oxide. Other were probably also converted into N-oxides. 4. oxidized secondary amines. 5. only slightly inhibited by CO not at all KCN proadifen hydrochloride (SKF 525-A), but trimethylsulphonium chloride, tetramethylammonium 2,4-dichloro-6-phenylphenoxyethylamine (Lilly 53325) its NN-diethyl derivative 18947). 6. showed similar response inhibitors parallel loss in activity heating 35°C. 7. activities the N-oxide demethylase secondary-amine mono-oxygenase increased severalfold during adaptation succinate-grown bacteria growth trimethylamine, first show an increase activity. It is concluded that three enzymes are involved this organism.