Evaluation of Three Methods for Discrimination of Bacillus anthracis From Other Bacillus Species
作者： Diane L Dutt , Jose-Luis Sagripanti , James Rogers
DOI:
关键词:
摘要: Abstract : Bacillus anthracis shares the same ecological niche with other members of B. cereus group: especially and thuringiensis. Techniques that differentiate among species using metabolic characteristics can be used to compliment PCR-based methods. These techniques include fatty acid profiling. In this work we compared two metabolicbased assays one profiling method for their effectiveness in distinguishing from spp. Our results indicate methods tested vary widely ability identify many strains.
参考文章(10)
P. Keim, A. M. Klevytska, L. B. Price, J. M. Schupp, G. Zinser, K. L. Smith, M. E. Hugh-Jones, R. Okinaka, K. K. Hill, P. J. Jackson, Molecular diversity in Bacillus anthracis Journal of Applied Microbiology. ,vol. 87, pp. 215- 217 ,(1999) , 10.1046/J.1365-2672.1999.00873.X
P. C. B. Turnbull, Definitive identification of Bacillus anthracis—a review Journal of Applied Microbiology. ,vol. 87, pp. 237- 240 ,(1999) , 10.1046/J.1365-2672.1999.00876.X
Jeffrey S Buyer, Rapid sample processing and fast gas chromatography for identification of bacteria by fatty acid analysis Journal of Microbiological Methods. ,vol. 51, pp. 209- 215 ,(2002) , 10.1016/S0167-7012(02)00081-7
L. V. H. MOORE, D. M. BOURNE, W. E. C. MOORE, Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. International Journal of Systematic and Evolutionary Microbiology. ,vol. 44, pp. 338- 347 ,(1994) , 10.1099/00207713-44-2-338
P. J. Jackson, K. K. Hill, M. T. Laker, L. O. Ticknor, P. Keim, Genetic comparison of Bacillus anthracis and its close relatives using amplified fragment length polymorphism and polymerase chain reaction analysis Journal of Applied Microbiology. ,vol. 87, pp. 263- 269 ,(1999) , 10.1046/J.1365-2672.1999.00884.X
M. A. Lee, G. Brightwell, D. Leslie, H. Bird, A. Hamilton, Fluorescent detection techniques for real-time multiplex strand specific detection of Bacillus anthracis using rapid PCR Journal of Applied Microbiology. ,vol. 87, pp. 218- 223 ,(1999) , 10.1046/J.1365-2672.1999.00908.X
Erlendur Helgason, Ole Andreas Økstad, Dominique A. Caugant, Henning A. Johansen, Agnes Fouet, Michéle Mock, Ida Hegna, Anne-Brit Kolstø, Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis—One Species on the Basis of Genetic Evidence Applied and Environmental Microbiology. ,vol. 66, pp. 2627- 2630 ,(2000) , 10.1128/AEM.66.6.2627-2630.2000
P. Keim, L. B. Price, A. M. Klevytska, K. L. Smith, J. M. Schupp, R. Okinaka, P. J. Jackson, M. E. Hugh-Jones, Multiple-Locus Variable-Number Tandem Repeat Analysis Reveals Genetic Relationships within Bacillus anthracis Journal of Bacteriology. ,vol. 182, pp. 2928- 2936 ,(2000) , 10.1128/JB.182.10.2928-2936.2000
P Keim, A Kalif, J Schupp, K Hill, S E Travis, K Richmond, D M Adair, M Hugh-Jones, C R Kuske, P Jackson, Molecular evolution and diversity in Bacillus anthracis as detected by amplified fragment length polymorphism markers Journal of Bacteriology. ,vol. 179, pp. 818- 824 ,(1997) , 10.1128/JB.179.3.818-824.1997
Bochner Br, Identification of over 500 Gram-negative species by a single test panel. American clinical laboratory. ,vol. 10, pp. 14- ,(1991)