Osteogenin inhibits proliferation and stimulates differentiation in mouse osteoblast-like cells (MC3T3-E1)
作者： Slobodan Vukicevic , Frank P. Luyten , A.H. Reddi
DOI: 10.1016/0006-291X(90)90873-L
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摘要: Abstract Osteogenin, a novel bone differentiation factor, was recently purified and characterized. We examined its effect on the proliferation of MC3T3-E1 osteoblast-like cells. Cell inhibited first 48 h after addition osteogenin, this independent serum. Osteogenin did not influence cell morphology. Alkaline phosphatase promptly increased in dose time-dependent manner appeared to be specific. Treatment with TGF- β 1 resulted inhibition alkaline activity, reversed by osteogenin within h. cultures treated for 72 confluence became responsive parathyroid hormone. Synthesis collagenous proteins stimulated osteogenin. The present results demonstrate significant osteogenic phenotype cells vitro.
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