Protocols for two- and three-color fluorescent RNA in situ hybridization of the main and accessory olfactory epithelia in mouse
作者： Tomohiro Ishii , Masayo Omura , Peter Mombaerts
DOI: 10.1007/S11068-005-3334-Y
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摘要: The main and accessory olfactory epithelia of the mouse are composed many cell populations. Each sensory neuron is thought to express one allele approximately 1000 odorant or 300 vomeronasal receptor genes. Sensory neurons die replaced by new that differentiate from precursor cells throughout lifetime individual. Neuronal replacement asynchronous, resulting in co-existence at various stages differentiation. Receptor gene diversity ongoing neuronal differentiation produce complex mosaics expression within these epithelia. Accurate description patterns will facilitate understanding mechanisms choice Here we report a detailed protocol for two- three-color fluorescent RNA situ hybridization (ISH) its combination with immunohistochemistry, detection bromodeoxyuridine (BrdU)-incorporated DNA after labeling. applied cryosections mouse.
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