Genomic organization of the human phosphodiesterase PDE11A gene

摘要: PDE11A is a dual-substrate, cAMP and cGMP, cyclic nucleotide phosphodiesterase (PDE). Presently four unique variants carrying distinct GAF sequences in the N-terminal region have been identified. While human PDE11A3 PDE11A4 are known to be specifically expressed testis prostate, respectively, PDE11A1 was mainly detected skeletal muscle. The gene investigated revealed span > 300 kb, contain 23 exons mapped on chromosome 2q31. transcription start sites of PDE11A1, were determined, promoter revealed. Although 5' flanking genomic regions had consensus TATA motif, that TATA-less but contained CCAAT box Sp1-binding sequence. Interestingly, we found exon 2 sequence for encoded an cytochrome c pseudogene alternate reading frame, C-terminal intron disrupted by insertion Alu repetitive Furthermore, examined exon-intron organization PDE2A compared among GAF-PDE family. catalytic domain very similar those PDE5A PDE6B. However, other GAF-PDEs, PDE10A, displayed different from although these three PDEs their amino-acid each other. findings suggested has common ancestral with PDE6s, whereas PDE10A generated separately GAF-PDEs.