Fourier Transform Infrared Spectroscopy Typing an Enterococcus sp.
作者： Mohammad I. Abu Taha , Hatem K. Eideh , Sameeh M. Saed , Hazem Jaber
关键词:
摘要: Enterococcus species are one of the leading causes nosocomial infections, which difficult to treat specially with rise its Vancomycin resistant. Studies isolates are essential for epidemiological investigation. Typing Enterococci is often based on traditional phenotypic as well as genotypic methods. In this study Fourier-transform infrared (FTIR) spectroscopy is used a novel approach typing Enterococci. FTIR spectroscopy results compared antibiotic susceptibility testing and PCR amplification of Vancomycin gene results; analysis showed that, 6 isolates were positive for Van (4 VanA, 1 VanB VanA plus VanB). Three plus VanB resistant all tested (Ampicillin, Teicoplamin Vancomycin) was found be sensitive. FTIR spectroscopy (first derivatives) divided into 8 groups. 3 groups isolates), (one isolate), VanB (one isolate) other 13 3 clusters. The demonstrated that spectroscopy has good discriminative capacity high reproducibility techniques.
sci-hub.st 参考文章(19)
Kazuo Nakamoto, Infrared spectra of inorganic and coordination compounds Wiley-Interscience. ,(1963)
K.C Schuster, F Mertens, J.R Gapes, FTIR SPECTROSCOPY APPLIED TO BACTERIAL CELLS AS A NOVEL METHOD FOR MONITORING COMPLEX BIOTECHNOLOGICAL PROCESSES Vibrational Spectroscopy. ,vol. 19, pp. 467- 477 ,(1999) , 10.1016/S0924-2031(98)00058-7
Dieter Naumann, Dieter Helm, Harald Labischinski, Microbiological characterizations by FT-IR spectroscopy Nature. ,vol. 351, pp. 81- 82 ,(1991) , 10.1038/351081A0
A. Alvarez-Ordóñez, M. Prieto, Changes in Ultrastructure and Fourier Transform Infrared Spectrum of Salmonella enterica Serovar Typhimurium Cells after Exposure to Stress Conditions Applied and Environmental Microbiology. ,vol. 76, pp. 7598- 7607 ,(2010) , 10.1128/AEM.00312-10
J. D. S. Goulden, M. E. Sharpe, The infra-red absorption spectra of lactobacilli. Microbiology. ,vol. 19, pp. 76- 86 ,(1958) , 10.1099/00221287-19-1-76
Shu-Xuan Deng, Jin-Long Yang, Ming-Shu Wang, An-Chun Cheng, Kang-Cheng Pan, Chuan-Feng Li, A simple and rapid method for extracting bacterial DNA from intestinal microflora for ERIC-PCR detection World Journal of Gastroenterology. ,vol. 14, pp. 2872- 2876 ,(2008) , 10.3748/WJG.14.2872
Dieter Helm, Harald Labischinski, Dieter Naumann, Elaboration of a procedure for identification of bacteria using Fourier-Transform IR spectral libraries: a stepwise correlation approach Journal of Microbiological Methods. ,vol. 14, pp. 127- 142 ,(1991) , 10.1016/0167-7012(91)90042-O
Barry J. Buschelman, Martha J. Bale, Ronald N. Jones, Species identification and determination of high-level aminoglycoside resistance among enterococci comparison study of sterile body fluid isolates, 1985–1991 Diagnostic Microbiology and Infectious Disease. ,vol. 16, pp. 119- 122 ,(1993) , 10.1016/0732-8893(93)90005-R
Ronald N Jones, Steven A Marshall, Michael A Pfaller, Werner W Wilke, Richard J Hollis, Meridith E Erwin, Michael B Edmond, Richard P Wenzel, SCOPE Hospital Study Group, Nosocomial enterococcal blood stream infections in the SCOPE program: Antimicrobial resistance, species occurrence, molecular testing results, and laboratory testing accuracy Diagnostic Microbiology and Infectious Disease. ,vol. 29, pp. 95- 102 ,(1997) , 10.1016/S0732-8893(97)00115-6
J.E.S. Greenstreet, K.P. Norris, The existence of differences between the infra-red absorption spectra of bacteria Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. ,vol. 9, pp. 177- 198 ,(1957) , 10.1016/0371-1951(57)80131-3