Intercellular communication in pancreatic islet monolayer cultures: a microfluorometric study
作者： E Kohen , C Kohen , B Thorell , D. Mintz , A Rabinovitch
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摘要: Single islet cells in monolayer cultures of neonatal rat pancreas were microinjected with fluorescein and scanned topographically by microfluorometry. Fluorescein spread from an injected cell directly into neighboring cells, and, the presence 16.7 millimolar glucose, significantly more communicated than glucose-free medium. Islet also glycolytic substrates activators that produced transient changes cellular levels reduced pyridine nucleotides-nicotinamide adenine dinucleotide nicotinamide phosphate [NAD(P)H]. Changes NAD(P)H fluorescence observed incubated first for 18 hours very low glucose concentrations then a medium activators; however, little change occurred adjacent cells. In contrast, after adding to medium, injection
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